In children, gastric washings/ aspirates may be collected. How is the sputum test performed?

A technician may visually check the color of the sputum that can give an idea about the type of the infection. In some cases, more than one sample may be needed.


Box 1 provides a reminder of the general principle of speci…
In order to produce a meaningful laboratory test result, follow these instructions exactly. A sputum stain for Mycobacteria is a laboratory test performed on a sample of your sputum, or phlegm. Obtaining a specimen involves collecting tissue or fluids for laboratory analysis or near-patient testing, and may be a first step in determining a diagnosis and treatment (Dougherty and Lister, 2015). Sputum induction is a procedure used for patients who have trouble producing sputum spontaneously. Ideally, the sample should be collected from a deep cough early in the morning.

[ 1] S The tests look for the presence of AFB bacteria in your sputum. A sputum culture requires minimal effort on your part. You’ll be asked to cough deeply to bring up the sputum … Sputum is material brought up from the lungs after a deep cough. Depending on symptoms, urine, an aspirate from the site of suspected infection, cerebrospinal fluid (CSF) , other body fluids , or biopsied tissue samples may be collected for AFB testing. The sputum that is coughed up by the patient is collected in a sterilized container for the necessary testing. Title: Sputum - CS - TB - AFB Doc.#38116 Ver 1.0 Page 1 of 1 Patient Instructions Sputum – C&S or TB/AFB Your health care provider has requested a sputum specimen(s) for the test(s) marked below [x]. [ ] C&S TEST: Collection 1. You might need to rinse your mouth with water first, and your doctor might ask you to skip a meal or stop taking any bacteria-killing antibiotics you’ve been given before the test. It’s also known as an acid-fast bacillus (AFB) stain or a tuberculosis (TB) smear. AFB tests are usually ordered for people with symptoms of active TB. You simply need to provide the sample for the lab to test. It is different from spit or saliva. If you are unable to produce sputum, a bronchoscope may be used to collect fluid during a procedure called a bronchoscopy. Specimens must be collected at the right time, using the correct technique and equipment, and be delivered to the laboratory in a timely manner (Dougherty and Lister, 2015). The patient inhales nebulised hypertonic saline solution, which liquefies airway secretions, promotes coughing and allows expectoration of respiratory secretions. Sputum is a thick mucus that is coughed up from the lungs.


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